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CRELD2 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37.5 kDa and the accession number is Q9CYA0.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $600 | 7-10 days |
Biological Activity | Measured by the ability of the immobilized protein to support the adhesion of ATDC5 mouse chondrogenic cells. When 5 x 10^4 cells/well are added to mCRELD2-coated plates (0.625 μg/mL, 100 μL/well),approximately 20% cells will adhere after 60 minutes at 37℃. |
Description | CRELD2 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37.5 kDa and the accession number is Q9CYA0. |
Species | Mouse |
Expression System | HEK293 Cells |
Tag | C-His |
Accession Number | Q9CYA0 |
Synonyms | cysteine-rich with EGF-like domains 2,C85758,5730592L21Rik |
Construction | A DNA sequence encoding the mouse CRELD2 (NP_083996.1) (Met1-Leu350) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Ser 23 |
Protein Purity | > 95 % as determined by SDS-PAGE |
Molecular Weight | 37.5 kDa (predicted); 51.3 kDa (reducing conditions) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
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