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CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His)

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CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His)

Catalog No. TMPY-05346

CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 163 kDa and the accession number is Q99ZW2.

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Pack Size	Price	Availability	Quantity
200 μg	$386	In Stock

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Biological Description

Biological Information	1 μg = 5 U1 U is defined as cutting 1 ug 1 KB dsDNA in 30 min at 37℃
Description	CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 163 kDa and the accession number is Q99ZW2.
Species	Streptococcus pyogenes
Expression System	Baculovirus Insect Cells
Tag	C-His
Accession Number	Q99ZW2
Synonyms	SpyCas9
Construction	The s. pyogenes serotype M1 cas9 (WP_010922251.1) (Met1-Asp1368) was expressed with a nuclear localization sequence (NLS) at the N-terminus, two NLSs and a polyhistidine tag at the C-terminus.
Protein Purity	≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Molecular Weight	163 kDa (predicted)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied from sterile 20 mM Tris, 300 mM NaCl, 50% glycerol, pH 8.0, 0.5 mM PMSF, 0.1 mM EDTA, 0.5 mM TCEP.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.