DBI Protein, Human, Recombinant (His)

Catalog No. TMPY-03991

The diazepam binding inhibitor (DBI), alternatively known as the acyl-CoA binding protein (ACBP), is involved in multiple biological actions. The polypeptide binds to the peripheral, or mitochondrial, benzodiazepine receptor and facilitates transport of cholesterol to the inner membrane to stimulate steroid synthesis. Through this action, DBI indirectly modulates gamma-aminobutyric acid (GABA)-mediated inhibitory neurotransmission. DBI can be postulated as a candidate gene for psychiatric phenotypes including anxiety, mood, and psychotic disorders. Diazepam Binding Inhibitor (DBI), also called acyl-CoA binding protein (ACBP), is a ubiquitously expressed protein originally identified based on its ability to displace diazepam from its binding site on the GABAA receptor. The mutant allele of the DBI was one of the risk factors for alcohol dependence as for the rs2276596 polymorphism.

DBI Protein, Human, Recombinant (His)

Catalog No. TMPY-03991

Pack Size	Price	Availability	Quantity
100 μg	$700	7-10 days

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QHow are recombinant protein products shipped?

AMost recombinant proteins are delivered as lyophilized powder, which are insensitive to temperature change and are stable at room temperature. They are typically shipped at room temperature or with blue ice. For liquid recombinant proteins, dry ice is generally used for shipping. It is normal if the product is arrived with a melted ice pack. The protein can still be used without any negative impact on its quality for a short period. If a specific protein is unstable at room temperature, appropriate cold chain shipping will be arranged to ensure product quality remains intact.

QCan protein activity be guaranteed?

AFor our in-stock products, activity data will be displayed on our official website. However, the absence of data does not mean there is no activity. It could be that we have not yet established an activity detection method or conducted testing. Nevertheless, our products are prioritized for expression and purification using methods known to retain activity.

QWhether recombinant proteins be available?

AWhat species of protein do you need, and do you have any requirements for expression systems and tags? What experiments will the product be used for?

QWhy are carrier proteins needed in some recombinant protein solutions?

ACarrier proteins help improve the stability of proteins and prevent them from adhereing to the walls upon freeze and thaw. If proteins adhere to the tube, it will lead to a decrease in protein concentration in the solution which affects protein activity. For long term storage, carrier proteins, such as 0.1% Bovine Serum Albumin (BSA), 5% Human Serum Albumin (HAS), 10% Fetal Bovine Serum (FBS), or 5% trehalose, are used to reduce such loss. If the concentration of the recombinant protein is low, please add an appropriate amount of carrier protein to maintain protein activity.

QHow to reconstitute lyophilized recombinant proteins into a stock solution?

ACentrifuge the product at 10,000-12,000 rpm for 20-30 seconds before opening the vial to drive any protein that may be lodged in the cap or on the side of the tube to the bottom of the vial.In general, most proteins can be reconstituted by simply adding sterile distilled water. If other diluents are needed, they will be specified in the product's Certificate of Analysis (COA). It is recommended to prepare the stock solution with a concentration not lower than 100 μg/mL and aliquot for storage to avoid repeated freeze-thaw cycles.

Biological Activity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	The diazepam binding inhibitor (DBI), alternatively known as the acyl-CoA binding protein (ACBP), is involved in multiple biological actions. The polypeptide binds to the peripheral, or mitochondrial, benzodiazepine receptor and facilitates transport of cholesterol to the inner membrane to stimulate steroid synthesis. Through this action, DBI indirectly modulates gamma-aminobutyric acid (GABA)-mediated inhibitory neurotransmission. DBI can be postulated as a candidate gene for psychiatric phenotypes including anxiety, mood, and psychotic disorders. Diazepam Binding Inhibitor (DBI), also called acyl-CoA binding protein (ACBP), is a ubiquitously expressed protein originally identified based on its ability to displace diazepam from its binding site on the GABAA receptor. The mutant allele of the DBI was one of the risk factors for alcohol dependence as for the rs2276596 polymorphism.
Species	Human
Expression System	E. coli
Tag	N-His
Accession Number	P07108-2
Synonyms	diazepam binding inhibitor (GABA receptor modulator, acyl-CoA binding protein),CCK-RP,ACBP,ACBD1
Construction	A DNA sequence encoding the human DBI (P07108-2) (Met1-Ala87) was expressed with a polyhistidine tag at the N-terminus. Predicted N terminal: His
Protein Purity	> 95 % as determined by SDS-PAGE
Molecular Weight	14 kDa (predicted); 18 kDa (reducing conditions)
Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The diazepam binding inhibitor (DBI), alternatively known as the acyl-CoA binding protein (ACBP), is involved in multiple biological actions. The polypeptide binds to the peripheral, or mitochondrial, benzodiazepine receptor and facilitates transport of cholesterol to the inner membrane to stimulate steroid synthesis. Through this action, DBI indirectly modulates gamma-aminobutyric acid (GABA)-mediated inhibitory neurotransmission. DBI can be postulated as a candidate gene for psychiatric phenotypes including anxiety, mood, and psychotic disorders. Diazepam Binding Inhibitor (DBI), also called acyl-CoA binding protein (ACBP), is a ubiquitously expressed protein originally identified based on its ability to displace diazepam from its binding site on the GABAA receptor. The mutant allele of the DBI was one of the risk factors for alcohol dependence as for the rs2276596 polymorphism.

DBI Protein, Human, Recombinant (His)

DBI Protein, Human, Recombinant (His)

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