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Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E/Envelope Protein (aa 247-675, His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 49.2 kDa and the accession number is AAC59274.1.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $451 | In Stock | |
200 μg | $771 | 7-10 days | |
500 μg | $1,560 | 7-10 days |
Biological Activity | Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
Description | Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E/Envelope Protein (aa 247-675, His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 49.2 kDa and the accession number is AAC59274.1. |
Species | DENV |
Expression System | Baculovirus Insect Cells |
Tag | C-His |
Accession Number | AAC59274.1 |
Synonyms | E Protein, DENV |
Construction | A DNA sequence encoding the envelope protein of Dengue virus (DENV)(type 2, strain New Guinea C/PUO-218 hybrid)(AAC59274.1) (Phe247-Gly675, with two amino acids "MV" at the N-terminal) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Met |
Protein Purity | > 80 % as determined by SDS-PAGE. |
Molecular Weight | 49.2 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 8.0, 10% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
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