Measured by its binding ability in a functional ELISA. Immobilized human EFNB2 at 2 μg/ml (100 μl/well) can bind human EphB4-Fc with a linear range of 1.56-12.5 ng/ml.

EphB4 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 83.8 kDa and the accession number is P54760.

Human

HEK293 Cells

TYRO11,MYK1,HTK,EPH receptor B4

A DNA sequence encoding the extracellular domain (Met 1-Ala 539) of human EphB4 (NP_004435.3) precursor was expressed with the fused Fc region of human IgG1 at the C-terminus. Predicted N terminal: Leu 16

> 90 % as determined by SDS-PAGE

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.