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Epstein-Barr virus (EBV) (strain B95-8) gH & gL Heterodimer Protein (His & Flag)

Catalog No. TMPY-06899

Epstein-Barr virus (EBV) (strain B95-8) gH & gL Heterodimer Protein (His & Flag) is expressed in HEK293 mammalian cells with His and Flag tag. The predicted molecular weight is 89.51 kDa and the accession number is P03231.

Epstein-Barr virus (EBV) (strain B95-8) gH & gL Heterodimer Protein (His & Flag)

Epstein-Barr virus (EBV) (strain B95-8) gH & gL Heterodimer Protein (His & Flag)

Catalog No. TMPY-06899
Epstein-Barr virus (EBV) (strain B95-8) gH & gL Heterodimer Protein (His & Flag) is expressed in HEK293 mammalian cells with His and Flag tag. The predicted molecular weight is 89.51 kDa and the accession number is P03231.
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100 μg$7007-10 days
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Product Information

Biological Activity
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
Epstein-Barr virus (EBV) (strain B95-8) gH & gL Heterodimer Protein (His & Flag) is expressed in HEK293 mammalian cells with His and Flag tag. The predicted molecular weight is 89.51 kDa and the accession number is P03231.
Species
EBV
Expression System
HEK293 Cells
TagC-His, C-Flag
Accession NumberP03231
Construction
A DNA sequence encoding the (Ser19-Leu682) of Epstein-Barr virus (EBV) (strain B95-8) gH (P03231) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 ; A DNA sequence encoding the (Asn23-Gly137) of Epstein-Barr virus (EBV) (strain B95-8) gL (P03212) was fused with a flag tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified Predicted N terminal: Ser 19 & Asn 23
Protein Purity
≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
Molecular Weight89.51 kDa (predicted); 79.81 kDa (reducing conditions)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.

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