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Epstein-Barr virus (EBV) (strain B95-8) gH & gL Heterodimer Protein (His & Flag) is expressed in HEK293 mammalian cells with His and Flag tag. The predicted molecular weight is 89.51 kDa and the accession number is P03231.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $700 | 7-10 days |
Biological Activity | Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
Description | Epstein-Barr virus (EBV) (strain B95-8) gH & gL Heterodimer Protein (His & Flag) is expressed in HEK293 mammalian cells with His and Flag tag. The predicted molecular weight is 89.51 kDa and the accession number is P03231. |
Species | EBV |
Expression System | HEK293 Cells |
Tag | C-His, C-Flag |
Accession Number | P03231 |
Construction | A DNA sequence encoding the (Ser19-Leu682) of Epstein-Barr virus (EBV) (strain B95-8) gH (P03231) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 ; A DNA sequence encoding the (Asn23-Gly137) of Epstein-Barr virus (EBV) (strain B95-8) gL (P03212) was fused with a flag tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified Predicted N terminal: Ser 19 & Asn 23 |
Protein Purity | ≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. |
Molecular Weight | 89.51 kDa (predicted); 79.81 kDa (reducing conditions) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
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