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FGF-2 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-00210

FGF-2 Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 18.5 kDa and the accession number is P15655.

FGF-2 Protein, Mouse, Recombinant (His)

FGF-2 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-00210
FGF-2 Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 18.5 kDa and the accession number is P15655.
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100 μg$245In Stock
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Product Information

Biological Activity
Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.1-0.6 ng/mL.
Description
FGF-2 Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 18.5 kDa and the accession number is P15655.
Species
Mouse
Expression System
E. coli
TagN-His
Accession NumberP15655
Synonyms
fibroblast growth factor 2 (basic),Fgfb,Fgf-2,bFGF
Construction
A DNA sequence encoding the mouse Fgf2 (NP_032032.1) (Ala11-Ser154) was expressed with a polyhistidine tag at the N-terminus. Predicted N terminal: Met
Protein Purity
> 95 % as determined by SDS-PAGE.
Molecular Weight18.5 kDa (predicted)
EndotoxinPlease contact us for more information.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI). bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies.bFGF/FGF2 Protein & Antibody Products

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Please read the User Guide of Recombinant Proteins for more specific information.

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