Fibronectin Protein, Human, Recombinant (aa 1266-1356, hFc)

Catalog No. TMPK-01373

Fibronectin is a high molecular glycoprotein present in the blood, connective tissue and at cell surface. It is synthesized by many types of differentiated cells and is believed to be involved in the attachment of cells to the surrounding extracellular matrix. Fibronectin has affinity to the other main components of extracellular matrix, collagen and glycosaminoglycans. Fibronectin Protein, Human, Recombinant (aa 1266-1356, hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 36.47 kDa and the accession number is P02751-13.

Fibronectin Protein, Human, Recombinant (aa 1266-1356, hFc)

Catalog No. TMPK-01373

Pack Size	Price	Availability
100 μg	$465	7-10 days
500 μg	$1,850	7-10 days
1 mg	$3,100	7-10 days

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Questions

QWhether recombinant proteins be available?

AWhat species of protein do you need, and do you have any requirements for expression systems and tags? What experiments will the product be used for?

QCan protein activity be guaranteed?

AFor our in-stock products, activity data will be displayed on our official website. However, the absence of data does not mean there is no activity. It could be that we have not yet established an activity detection method or conducted testing. Nevertheless, our products are prioritized for expression and purification using methods known to retain activity.

QHow to reconstitute lyophilized recombinant proteins into a stock solution?

ACentrifuge the product at 10,000-12,000 rpm for 20-30 seconds before opening the vial to drive any protein that may be lodged in the cap or on the side of the tube to the bottom of the vial.In general, most proteins can be reconstituted by simply adding sterile distilled water. If other diluents are needed, they will be specified in the product's Certificate of Analysis (COA). It is recommended to prepare the stock solution with a concentration not lower than 100 μg/mL and aliquot for storage to avoid repeated freeze-thaw cycles.

QHow to store the recombinant protein products?

A1. It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, they should be used promptly. If they cannot be used up within one week, the product can be stored at -20°C to -80°C for at least 3 months. 2. Avoid multiple freeze-thaw cycles and store products in aliquots. When aliquoting and freezing, it is advisable to use a solution or medium with a certain concentration of carrier protein for reconstitution. The product concentration should not be less than 100 μg/mL, and the volume per vial should be no less than 20 μL.

QWhy are carrier proteins needed in some recombinant protein solutions?

ACarrier proteins help improve the stability of proteins and prevent them from adhereing to the walls upon freeze and thaw. If proteins adhere to the tube, it will lead to a decrease in protein concentration in the solution which affects protein activity. For long term storage, carrier proteins, such as 0.1% Bovine Serum Albumin (BSA), 5% Human Serum Albumin (HAS), 10% Fetal Bovine Serum (FBS), or 5% trehalose, are used to reduce such loss. If the concentration of the recombinant protein is low, please add an appropriate amount of carrier protein to maintain protein activity.

Biological Activity	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	Fibronectin is a high molecular glycoprotein present in the blood, connective tissue and at cell surface. It is synthesized by many types of differentiated cells and is believed to be involved in the attachment of cells to the surrounding extracellular matrix. Fibronectin has affinity to the other main components of extracellular matrix, collagen and glycosaminoglycans. Fibronectin Protein, Human, Recombinant (aa 1266-1356, hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 36.47 kDa and the accession number is P02751-13.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	P02751-13
Synonyms	SMDCF,MSF,LETS,GFND2,GFND,FNZ,FN1,FN,FINC,ED-B,CIG
Construction	Glu1266-Thr1356
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	36.47 kDa (predicted). Due to glycosylation, the protein migrates to 47-57 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Fibronectin is a high molecular glycoprotein present in the blood, connective tissue and at cell surface. It is synthesized by many types of differentiated cells and is believed to be involved in the attachment of cells to the surrounding extracellular matrix. Fibronectin has affinity to the other main components of extracellular matrix, collagen and glycosaminoglycans.

Fibronectin Protein, Human, Recombinant (aa 1266-1356, hFc)

Fibronectin Protein, Human, Recombinant (aa 1266-1356, hFc)

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