1. Immobilized Recombinant Human AXL Protein (Fc Tag) at 2 μg/mL (100 μL/well) can bind GAS6 Protein, Human, Recombinant (aa 279-678, His) (Cat#TMPY-07110), the EC50 is 5-15 ng/mL.
2. AXL Protein, Human, Recombinant (mFc) (Cat#TMPY-06478) captured on CM5 chip via Anti-Mouse antibody can bind GAS6 Protein, Human, Recombinant (aa 279-678, His) (Cat#TMPY-07110) with an affinity constant of 4.277 nM as determined in an SPR assay (Biacore T200) (Routinely tested).

GAS6 Protein, Human, Recombinant (aa 279-678, His) is expressed in HEK293 Cells. The accession number is Q14393-2.

Human

HEK293 Cells

FLJ34709,DKFZp666G247,AXSF,AXLLG

A DNA sequence encoding the Human GAS6 (Q14393-2) (Asp279-Ala678) was expressed with a polyhistidine tag at the C-terminus.

≥ 95% as determined by SDS-PAGE. ≥ 95% as determined by SEC-HPLC.

Please refer to the lot-specific COA.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. After reconstitution under sterile conditions, samples are stable for up to three months at -20℃ to -80℃.

The growth arrest-specific 6 gene (GAS6) is a member of the family of plasma vitamin K-dependent proteins, which are able to bind to phospholipids using an N-terminal gamma-carboxyglutamic acid domain. GAS6 is a vitamin K-dependent protein, plays a role in the survival, proliferation, migration, differentiation, adhesion, and apoptosis of cells. The growth arrest-specific 6 (GAS6) has been implicated in systemic inflammation and coagulation. Growth arrest-specific 6 (GAS6), plays a role in tumor progression by regulating growth in many cancers. GAS6, expressed by osteoblasts in the bone marrow, plays a significant role in the regulation of PCa cell survival during chemotherapy, which will have important implications for targeting metastatic disease. The GAS6/TYRO3-AXL-MERTK (TAM) signaling pathway is essential for full and sustained platelet activation, as well as thrombus stabilization. Inhibition of this pathway decreases platelet aggregation, shape change, clot retraction, aggregate formation under flow conditions, and surface expression of activation markers. It had been show that GAS6 signaling regulates invasion, proliferation, chemotherapy-induced apoptosis of prostate cancer (PCa) cells, and GAS6 secreted from osteoblasts in the bone marrow environment plays a critical role in establishing prostate tumor cell dormancy.