Immobilized Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&AFP (FMNKFIYEI) Antibody, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA.Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer, His Tag captured on CM5 Chip via Anti-His Antibody can bind HLA-A*02:01&B2M&AFP (FMNKFIYEI) TCR with an affinity constant of 0.923 μM as determined in SPR assay (Biacore T200).

Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins.CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response.

Human

HEK293 Cells

HPAFP,Alpha-fetoprotein,MHC,FETA,Alpha-feto,Alpha-1-fetoprotein,AFPD,AFP

Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and FMNKFIYEI peptide

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins.CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response.