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Structural protein that is not essential for the viral replication either in tissue culture or in its natural host. Human coronavirus HKU1 (isolate N5) Protein I (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 27.8 kDa and the accession number is Q0ZME2.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μg | $198 | 20 days | |
100 μg | $389 | 20 days | |
1 mg | $1,680 | 20 days |
Biological Activity | Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
Description | Structural protein that is not essential for the viral replication either in tissue culture or in its natural host. Human coronavirus HKU1 (isolate N5) Protein I (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 27.8 kDa and the accession number is Q0ZME2. |
Species | HCoV-HKU1 |
Expression System | E. coli |
Tag | N-10xHis, C-Myc |
Accession Number | Q0ZME2 |
Synonyms | Protein in nucleocapsid ORF,Protein I,Orf8 protein,N internal ORF protein,N,Accessory protein N2 |
Amino Acid | MLEVEAPLEIVQESSRKLLGLTNLSEAIKPIIEAENPNQNSLCLLNHKETLSHIIPGSLGLPNFKKVETLNFQMVKEYPLLTGYPLLKQKDIGINTTGVLLKQLMVNKSSCYQDGISTISVPVHMPVHPMVMPTKVSSGSLVTKLILLFPPMFRQGILLFKKLSLLGFRLVRFCLKAIMLKAQEGLLLIAGQVHVLNHVDPIIVH |
Construction | 1-205 aa |
Protein Purity | > 85% as determined by SDS-PAGE. |
Molecular Weight | 27.8 kDa (predicted) |
Formulation | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution | Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
Research Background | Structural protein that is not essential for the viral replication either in tissue culture or in its natural host. |
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