IFN-alpha 1/IFNA1 Protein, Human, Recombinant (hFc)

Catalog No. TMPK-00075

IFN-α, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes. IFN-alpha 1/IFNA1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 46.14 kDa and the accession number is P01562.

IFN-alpha 1/IFNA1 Protein, Human, Recombinant (hFc)

Catalog No. TMPK-00075

Pack Size	Price	Availability
100 μg	$487	7-10 days
500 μg	$1,950	7-10 days
1 mg	$3,250	7-10 days

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User Guide of Recombinant Proteins

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Product Information

Biological Activity	Human IFN alpha/beta R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 0.12 μM as determined in SPR assay.
Description	IFN-α, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes. IFN-alpha 1/IFNA1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 46.14 kDa and the accession number is P01562.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	P01562
Synonyms	LeIF D,Interferon α-D,Interferon α-1/13,Interferon α1,Interferon alpha-D,Interferon alpha-1/13,Interferon alpha 1,IFN-α-1/13,IFN-α-1,IFNα1,IFN-alpha-1/13,IFN-alpha-1,IFNalpha 1,IFNA1,IFN α1,IFN α 1,IFN alpha1
Construction	Cys24-Glu189
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	46.14 kDa (predicted). Due to glycosylation, the protein migrates to 48-55 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	IFN-α, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes.