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IFN-alpha 2b/IFNA2 Protein, Human, Recombinant (His & Avi), Biotinylated

Catalog No. TMPY-07119

IFN-alpha 2b/IFNA2 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is P01563-1.

IFN-alpha 2b/IFNA2 Protein, Human, Recombinant (His & Avi), Biotinylated

IFN-alpha 2b/IFNA2 Protein, Human, Recombinant (His & Avi), Biotinylated

Catalog No. TMPY-07119
IFN-alpha 2b/IFNA2 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is P01563-1.
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20 μg $462Backorder
100 μg $1,060Backorder
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Product Information

Biological Activity
Immobilized IFN-alpha 2b/IFNA2 Protein, Human, Recombinant (His & Avi), Biotinylated (Cat#TMPY-07119) at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind IFNAR2 Protein, Human, Recombinant (hFc) (Cat#TMPY-01640), the EC50 is 12-40 ng/mL.
Description
IFN-alpha 2b/IFNA2 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is P01563-1.
Species
Human
Expression System
HEK293 Cells
TagC-His-Avi
Accession NumberP01563-1
Synonyms
leIF A,IFN-alphaA,IFN-alpha-2,IFNA2C,IFNA2B,IFNA2A,IFNA
Construction
A DNA sequence encoding the Human Interferon alpha 2 / IFNA2 (P01563-1) (Met1-Glu188) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Protein Purity
≥ 95% as determined by SDS-PAGE. ≥ 90% as determined by SEC-HPLC.
Molecular Weight22.53 kDa (predicted); 25.1 kDa and 23.5 kDa (reducing contition, due to glycosylation)
Endotoxin< 1.0 EU per μg of the protein as determined by the LAL method
FormulationLyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Please refer to the lot-specific COA.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
IFNA2 (Interferon Alpha 2) is a Protein Coding gene. This gene is a member of the alpha interferon gene cluster on chromosome 9. The encoded protein is a cytokine produced in response to viral infection. Type I Interferons (IFNs) are well-known cytokines that exert antiviral activity, antitumor activity, and immunomodulatory effects. Interferon tau (IFNT), a type I IFN similar to alpha IFNs (IFNA), is the pregnancy recognition signal produced by the ruminant conceptus. Among the IFN-α genes, a total of 28 different sequence variants have been described. The three principal subtypes of IFNα-2 are designated α-2a, α-2b, and α-2c. IFNα-2b is being the predominant allele while IFNα-2a is less predominant and IFNα-2c only a minor allelic variant.

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Please read the User Guide of Recombinant Proteins for more specific information.