Interferon alpha 4/IFNA4 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-03892

Interferon alpha 4/IFNA4 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 20.2 kDa and the accession number is P07351.

Interferon alpha 4/IFNA4 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-03892

Interferon alpha 4/IFNA4 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 20.2 kDa and the accession number is P07351.

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Product Information

Biological Activity	Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 10-50 pg/mL.
Description	Interferon alpha 4/IFNA4 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 20.2 kDa and the accession number is P07351.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P07351
Synonyms	RP23-139P14.30-001,MGC143607,interferon, α4,interferon, alpha 4,Ifa4
Construction	A DNA sequence encoding the mouse IFNA4 (NP_034634.1) (Met1-Glu186) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Cys 25
Protein Purity	> 95 % as determined by SDS-PAGE
Molecular Weight	20.2 kDa (predicted); 22-27 kDa (reducing conditions)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Interferon, alpha 4 (IFNA4) belongs to the alpha/beta interferon family. Two variants of IFNA4 (IFNA4a and IFNA4b) are known, which differ from each other by changes in their coding regions at nucleotide positions 220 and 410 and can be distinguished by selective restriction enzyme analysis. Interferons are produced by macrophages, IFN-alpha has antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha, the first cytokine to be produced by recombinant DNA technology, has emerged as an important regulator of growth and differentiation, affecting cellular communication and signal transduction pathways as well as immunological control. Originally discovered as an antiviral substance, the efficacy of IFN-alpha in malignant, viral, immunological, angiogenic, inflammatory, and fibrotic diseases suggests a spectrum of interrelated pathophysiologies. IFN-alpha emerged as a prototypic tumor suppressor protein that represses the clinical tumorigenic phenotype in some malignancies capable of differentiation.