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IGFBP-5 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 30 kDa and the accession number is P24593.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $451 | In Stock | |
200 μg | $798 | 7-10 days | |
500 μg | $1,690 | 7-10 days |
Biological Activity | Measured by its ability to inhibit the biological activity of IGFI or IGFII on MCF7 human breast cancer cells. The ED50 for this effect is 0.1-0.5μg/mL in the presence of 14 ng/mL rhIGFII. |
Description | IGFBP-5 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 30 kDa and the accession number is P24593. |
Species | Human |
Expression System | Baculovirus Insect Cells |
Tag | C-His |
Accession Number | P24593 |
Synonyms | insulin-like growth factor binding protein 5,IBP5 |
Construction | A DNA sequence encoding the human IGFBP5 (NP_000590.1 ) (Met1-Glu272) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Leu 21 |
Protein Purity | > 95 % as determined by SDS-PAGE. |
Molecular Weight | 30 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, pH 8.0, 300 mM NaCl, 10% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
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