1. Loaded Biotinylated Human IgG1 Fc-His-Avi on SA Biosensor, can bind Protein G-Fc with an affinity constant of <1 pM as determined in BLI assay.  (Regularly tested) 2. Immobilized Biotinylated Human IgG1 Fc at 2μg/ml (100 μl/well) can bind Recombinant Protein G. The ED50 of Recombinant Protein G is 49.41 ng/ml. (Regularly tested)

IgG1 hFc Protein, Human, Recombinant, Biotinylated is expressed in HEK293 mammalian cells with C-Avi tag. The predicted molecular weight is 33 KDa and the accession number is P01857.

Human

HEK293 Cells

IGHG1,g γ-1 chain C region,g gamma-1 chain C region

Asp104-Lys330(Asp239Glu,Leu241Glu)

Asp104-Lys330(Asp239Glu,Leu241Glu)

Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity.