IL-13RA2 Protein, Canine, Recombinant (His)

Catalog No. TMPY-03772

IL-13RA2 Protein, Canine, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 38.6 kDa and the accession number is Q95LF0.

IL-13RA2 Protein, Canine, Recombinant (His)

Catalog No. TMPY-03772

IL-13RA2 Protein, Canine, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 38.6 kDa and the accession number is Q95LF0.

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100 μg	$600	7-10 days

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User Guide of Recombinant Proteins

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Product Information

Biological Activity	Measured by its ability to inhibit IL13-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.01-0.04 μg/mL.
Description	IL-13RA2 Protein, Canine, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 38.6 kDa and the accession number is Q95LF0.
Species	Canine
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q95LF0
Synonyms	interleukin 13 receptor, α2,interleukin 13 receptor, alpha 2
Construction	A DNA sequence encoding the canine IL13RA2 (Q95LF0) (Met1-Thr338) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Ser 22
Protein Purity	> 95 % as determined by SDS-PAGE
Molecular Weight	38.6 kDa (predicted); 39-46 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Interleukin-13 receptor subunit alpha-2 (IL13RA2/IL-13RA2) is also known as cluster of differentiation 213A2 (CD213A2), IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, and IL-13RA2. The IL13RA2 is often overexpressed in brain tumors, making Il13ra2 one of the vaccine targets for immunotherapy of glioma. IL13RA2/IL-13RA2 is a cancer-associated receptor that is present in greater than 80% of High-Grade Astrocytomas (HGA) and has recently been recognized as a cytokine that predisposes breast cancer cells to metastasize. Expression of IL13Rα2 was rapidly lost from the surface of transduced cells grown in culture. The loss appeared to be related to ligands present in fetal bovine serum in the medium. None of the malignant glioma cell lines cultivated in vitro and tested to date exhibited the IL13Rα2 receptor. A recombinant virus (R5111) enters cells via its interaction with the IL13Rα2 receptor in a manner that cannot be differentiated from the interaction of wild-type virus with its receptors.