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IL-31 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 17.3 kDa and the accession number is Q6EBC2.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
10 μg | $106 | In Stock | |
20 μg | $159 | 7-10 days | |
50 μg | $267 | 7-10 days | |
100 μg | $397 | 7-10 days | |
200 μg | $700 | 7-10 days | |
500 μg | $1,480 | 7-10 days |
Biological Activity | Measured by its ability to induce STAT3 activation in U‑87 cells. The ED50 for this effect is 11-45 ng/mL. |
Description | IL-31 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 17.3 kDa and the accession number is Q6EBC2. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-His |
Accession Number | Q6EBC2 |
Synonyms | interleukin 31,IL-31 |
Construction | A DNA sequence encoding the human IL31 (NP_001014358.1) (Met1-Thr164) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Ser 24 |
Protein Purity | > 95 % as determined by SDS-PAGE |
Molecular Weight | 17.3 kDa (predicted); 31.8 kDa (reducing condition, due to glycosylation) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
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