Measured by its binding ability in a functional ELISA. Immobilized Canine IL33 at 10 μg/ml (100 μl/well) can bind human IL1R4-Fc , The EC50 of human IL1R4-Fc is 0.25-0.66 μg/ml.

IL-33 Protein, Canine, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 17.8 kDa and the accession number is O97863.

Canine

E. coli

interleukin 33

The mature form of canine IL33 (O97863) (Ser 110-Ser 263) was expressed, with an initial Met at the N-terminus.

> 97 % as determined by SDS-PAGE

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Interleukin 33 (IL-33), also known as DVS27 or NF-HEV (Nuclear Factor from High Endothelial Venules), is a pro-inflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 protein is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyer's patches, and lymph nodes. IL-33 protein has transcriptional regulatory properties, and the researches suggested that IL-33 is a dual-function protein that might act both as a cytokine and as an intracellular nuclear factor. As a type 2 cytokines, IL-33 protein also play a pivotal role in helminthic infection and allergic disorders.