1. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-1 ng/mL.
2. Loaded IL-5R alpha/CD125 Protein, Human, Recombinant (Membrane-bound, His) (Cat#TMPY-07016) on HIS1K Biosensor, can bind IL-5 Protein, Human, Recombinant (HEK293) (Cat#TMPY-07129) with an affinity constant of 43.200 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

IL-5 Protein, Human, Recombinant (HEK293) is expressed in HEK293 Cells. The accession number is P05113-1.

Human

HEK293 Cells

TRF,IL-5,EDF

A DNA sequence encoding the human IL5 (NP_000870.1) (Ile20-Ser134) was expressed.

> 95% as determined by SDS-PAGE.

Please refer to the lot-specific COA.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Interleukin 5 (IL-5) is a member of the interleukin family with a length of 115 amino acids. Interleukins are a group of cytokines (secreted proteins/signaling molecules) that were first seen to be expressed by white blood cells (leukocytes) and has been found in a wide variety of body cells. Interleukin 5 or IL-5 is produced by T helper-2 cells and mast cells. It helps to stimulate B cell growth and increase immunoglobulin secretion and is considered a key mediator in eosinophil activation. Interleukin 5 (IL-5) has long been associated with several allergic diseases, including allergic rhinitis and asthma. Growth in the number of circulating, airway tissue, and induced sputum eosinophils have been observed in patients with these diseases. IL-5 also had something with the terminally differentiated granulocyte eosinophils. IL-5 was originally found as an eosinophil colony-stimulating factor. It has been proved to be a major regulator of eosinophil accumulation in tissues and can modulate eosinophil behavior at every stage from maturation to survival.