1. Immobilized Recombinant Human IL-5 Protein (His & AVI Tag), Biotinylated at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind IL-5R alpha/CD125 Protein, Human, Recombinant (Membrane-bound, hFc) (Cat#TMPY-07015), the EC50 is 20-60 ng/mL.
2. Loaded Biotinylated Recombinant Human IL-5 Protein, His & AVI Tag on SA Biosensor, can bind IL-5R alpha/CD125 Protein, Human, Recombinant (Membrane-bound, hFc) (Cat#TMPY-07015) with an affinity constant of 2.320 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

IL-5R alpha/CD125 Protein, Human, Recombinant (Membrane-bound, hFc) is expressed in HEK293 Cells. The accession number is Q01344-1.

≥ 95% as determined by SDS-PAGE. ≥ 90% as determined by SEC-HPLC.

Interleukin 5 receptor, alpha (IL5RA) also known as CD125 (Cluster of Differentiation 125) is a subunit of the Interleukin-5 receptor. IL5RA (CD125) is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand-specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony-stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Six alternatively spliced transcript variants encoding three distinct isoforms have been reported. IL5RA (CD125) is a T-cell-derived cytokine that is particularly important in the development of asthma for the terminal differentiation, activation, and survival of committed eosinophil precursors.