IRE1 Protein, Human, Recombinant (aa 465-977)

Catalog No. TMPY-04753

IRE1 Protein, Human, Recombinant (aa 465-977) is expressed in Baculovirus insect cells. The predicted molecular weight is 58.3 kDa and the accession number is O75460-1.

IRE1 Protein, Human, Recombinant (aa 465-977)

Catalog No. TMPY-04753

IRE1 Protein, Human, Recombinant (aa 465-977) is expressed in Baculovirus insect cells. The predicted molecular weight is 58.3 kDa and the accession number is O75460-1.

Pack Size	Price	Availability
50 μg	$320	In Stock
100 μg	$547	7-10 days
200 μg	$935	7-10 days
500 μg	$1,890	7-10 days

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Product Information

Biological Activity	1. Kinase activity untested 2. Measured by its nuclease activity to cleave Xbp1 single stem-loop mini-substrate.
Description	IRE1 Protein, Human, Recombinant (aa 465-977) is expressed in Baculovirus insect cells. The predicted molecular weight is 58.3 kDa and the accession number is O75460-1.
Species	Human
Expression System	Baculovirus Insect Cells
Tag	Tag Free
Accession Number	O75460-1
Synonyms	IRE1P,IRE1a,IRE1,hIRE1p,endoplasmic reticulum to nucleus signaling 1
Construction	A DNA sequence encoding the human ERN1 (O75460-1) (Pro 465-Leu 977) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus. Predicted N terminal: Gly
Protein Purity	> 80 % as determined by SDS-PAGE
Molecular Weight	58.3 kDa (predicted); 65 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Shipping with blue ice.
Research Background	Endoplasmic reticulum stress and hypoxia are necessary components of malignant tumors growth and suppression of ERN1 (from endoplasmic reticulum to nuclei-1) signalling pathway, which is linked to the apoptosis and cell death processes, significantly decreases proliferative processes. An enhanced expression of TP53 gene in ERN1 knockdown glioma cells correlates with the decreased level of ubiquitin ligase MDM2 and increased expression level of USP7 which deubiquitinates TP53 and MDM2 and induces TP53-dependent cell growth repression and apoptosis. Thus, the expression of genes encoding TP53 and related to TP53 factors depends upon the endoplasmic reticulum stress signaling as well as on hypoxia, and correlates with suppression of glioma growth under ERN1 knockdown. The dependence of insulin-like growth binding proteins as well as IGF2BP3 and HTRA1 gene expressions in U87 glioma cells on ERN1 signaling enzyme function and hypoxia, indicating its participation in the regulation of metabolic and proliferative processes via IGF/INS receptors, because endoplasmic reticulum stress is an important component of tumor growth and metabolic diseases.