KEAP1 Protein, Human, Recombinant

Catalog No. TMPY-03432

KEAP1 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 69.7 kDa and the accession number is Q14145.

KEAP1 Protein, Human, Recombinant

Catalog No. TMPY-03432

KEAP1 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 69.7 kDa and the accession number is Q14145.

Pack Size	Price	Availability
100 μg	$386	In Stock
200 μg	$660	7-10 days
500 μg	$2,080	7-10 days

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COA User Guide of Recombinant Proteins

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Product Information

Biological Activity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	KEAP1 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 69.7 kDa and the accession number is Q14145.
Species	Human
Expression System	Baculovirus Insect Cells
Tag	Tag Free
Accession Number	Q14145
Synonyms	KLHL19,kelch like ECH associated protein 1,KEAP-1,INRF2
Construction	A DNA sequence encoding the human KEAP1 (Q14145) (Gln2-Cys624) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus. Predicted N terminal: Gly
Protein Purity	> 85 % as determined by SDS-PAGE
Molecular Weight	69.7 kDa (predicted); 64 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 3 mM DTT, 10% glycerol, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Kelch-like ECH-associated protein 1, also known as a cytosolic inhibitor of Nrf2, Kelch-like protein 19, KEAP1, and INRF2, is a cytoplasm and nucleus protein that contains one BACK (BTB/Kelch associated) domain, one BTB (POZ) domain, and six Kelch repeats. KEAP1 / INRF2 is broadly expressed, with the highest levels in skeletal muscle. KEAP1 / INRF2 is a key regulator of the NRF2 transcription factor, which transactivates the antioxidant response element (ARE) and upregulates numerous proteins involved in antioxidant defense. Under basal conditions, KEAP1 / INRF2 targets NRF2 for ubiquitination and proteolytic degradation and as such is responsible for the rapid turnover of NRF2. KEAP1 / INRF2 retains NFE2L2 / NRF2 in the cytosol. KEAP1 / INRF2 functions as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. It targets NFE2L2 / NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. KEAP1 / INRF2 may also retain BPTF in the cytosol. It targets PGAM5 for ubiquitination and degradation by the proteasome.