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Neurolysin Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 78.7 kDa and the accession number is Q9BYT8.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μg | $600 | 7-10 days |
Biological Activity | Measured by its ability to cleave a fluorogenic peptide substrate, (7-methoxycoumarin-4-yl)acetyl Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl)-OH. The specific activity is > 100 pmol/min/μg. |
Description | Neurolysin Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 78.7 kDa and the accession number is Q9BYT8. |
Species | Human |
Expression System | E. coli |
Tag | N-His |
Accession Number | Q9BYT8 |
Synonyms | NLN,neurolysin (metallopeptidase M3 family),MOP,MEP,KIAA1226,EP24.16,AGTBP |
Construction | A DNA sequence encoding the human NLN (NP_065777.1) (Ser38-Pro704) was expressed with a polyhistidine tag at the N-terminus. Predicted N terminal: His |
Protein Purity | > 90 % as determined by SDS-PAGE |
Molecular Weight | 78.7 kDa (predicted); 67 kDa (reducing conditions) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
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