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NGAL/Lipocalin-2 Protein, Human, Recombinant (hFc)

Catalog No. TMPK-00085

NGAL/Lipocalin-2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 47.2 kDa and the accession number is P80188-1.

NGAL/Lipocalin-2 Protein, Human, Recombinant (hFc)

NGAL/Lipocalin-2 Protein, Human, Recombinant (hFc)

Catalog No. TMPK-00085
NGAL/Lipocalin-2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 47.2 kDa and the accession number is P80188-1.
Pack SizePriceAvailabilityQuantity
100 μg $3027-10 days
500 μg $1,2107-10 days
1 mg $2,0107-10 days
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Product Information

Biological Activity
Immobilized Human NGAL, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-NGAL Antibody, hFc Tag with the EC50 19.7ng/ml determined by ELISA.
Description
NGAL/Lipocalin-2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 47.2 kDa and the accession number is P80188-1.
Species
Human
Expression System
HEK293 Cells
TagC-hFc
Accession NumberP80188-1
Synonyms
Siderocalin LCN2,p25,Oncogene 24p3,NGAL,MSFI,Lipocalin-2
Construction
Gln21-Gly198
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight47.2 kDa (predicted). Due to glycosylation, the protein migrates to 48-52 kDa based on Tris-Bis PAGE result.
Endotoxin< 1 EU/μg by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM MES, 150 mM NaCl (pH 6.5). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution
Reconstitute the lyophilized protein in 50mM MES,150mM NaCl (pH 6.5). The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
Acute kidney injury (AKI) is one of the most common complications of various serious conditions, and early diagnosis is therefore critical for the treatment of AKI. Recent evidence demonstrates that neutrophil gelatinase- associated lipocalin (NGAL) is closely associated with AKI. Several experimental and clinical studies have shown that the expression of urine and serum NGAL increases significantly in AKI. NGAL shows potential to be a new effective early biochemical marker of AKI. Further studies are needed to confirm the significant advantages of NGAL in the diagnosis of early AKI and its value in clinical applications.

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Please read the User Guide of Recombinant Proteins for more specific information.