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NKp80/KLRF1 Protein, Human, Recombinant (Active, hFc)

Catalog No. TMPY-07046

NKp80/KLRF1 Protein, Human, Recombinant (Active, hFc) is expressed in HEK293 Cells. The accession number is Q9NZS2-1.

NKp80/KLRF1 Protein, Human, Recombinant (Active, hFc)

NKp80/KLRF1 Protein, Human, Recombinant (Active, hFc)

Catalog No. TMPY-07046
NKp80/KLRF1 Protein, Human, Recombinant (Active, hFc) is expressed in HEK293 Cells. The accession number is Q9NZS2-1.
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Product Information

Biological Activity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells (Routinely tested).
Description
NKp80/KLRF1 Protein, Human, Recombinant (Active, hFc) is expressed in HEK293 Cells. The accession number is Q9NZS2-1.
Species
Human
Expression System
HEK293 Cells
TagN-hFc
Accession NumberQ9NZS2-1
Synonyms
NKp80,ML,CLEC5C
Construction
A DNA sequence encoding the Human KLRF1 (NP_057607.1) (Val66-Tyr231) was expressed with the Fc region of human IgG1 at the N-terminus.
Protein Purity
≥ 90% as determined by SDS-PAGE. ≥ 90% as determined by SEC-HPLC.
Molecular Weight45.79 kDa (predicted); 52.5 kDa (reducing contition, due to glycosylation)
Endotoxin< 1.0 EU per μg of the protein as determined by the LAL method
FormulationLyophilized from sterile 20 mM MES, 150 mM NaCl, 8% Glycerol, 10% trehalose, pH 6.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Please refer to the lot-specific COA.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
NKp80, also known as KLRF1, is an activating homodimeric C-type lectin-like receptor that is expressed on nearly all-natural killer cells and stimulates their cytotoxicity and cytokine release. NKp80 stimulates cytotoxicity upon engagement of its genetically linked ligand: myeloid-specific CTLR activation-induced C-type lectin (AICL). NKp80, but not NKp80 mutated at tyrosine 7 (NKp80/Y7F), is tyrosine phosphorylated. Accordingly, NKp80/Y7F, but not NKp80/Y3F or NKp80/Y37F, failed to induce cytotoxicity. NKp80 phosphopeptides comprising the Hemi-ITAM-like sequence surrounding tyrosine 7 bound Lck- and Syk-family kinases; accordingly, cross-linking of NKp8, but not NKp80/Y7F, induced Syk phosphorylation. Moreover, inhibition of Syk kinase, but not ZAP-7 kinase, impaired cytotoxic responses through NKp80. Atypical residues in the Hemi-ITAM-like motif of NKp80 cause an altered stoichiometry of phosphorylation but did not substantially affect NK cytotoxicity. Altogether, these results show that NKp80 uses an atypical Hemi-ITAM and Syk kinase to trigger cellular cytotoxicity.

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