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Nucleocapsid protein (N) is the major viral structural component; its main function is to protect and encapsidate the viral RNA forming viral RNP complex. It is encoded by the S segment vRNA and is abundantly expressed in the cytoplasm of infected cells. SARS-COV-2 Nucleocapsid Protein CTD Domain (His & Avi) is expressed in E. coli expression system with N-His-Avi tag. The predicted molecular weight is 17 kDa and the accession number is P0DTC9.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | 219 € | 7-10 days | |
500 μg | 885 € | 7-10 days | |
1 mg | 1.472 € | 7-10 days |
Biological Activity | Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
Description | Nucleocapsid protein (N) is the major viral structural component; its main function is to protect and encapsidate the viral RNA forming viral RNP complex. It is encoded by the S segment vRNA and is abundantly expressed in the cytoplasm of infected cells. SARS-COV-2 Nucleocapsid Protein CTD Domain (His & Avi) is expressed in E. coli expression system with N-His-Avi tag. The predicted molecular weight is 17 kDa and the accession number is P0DTC9. |
Species | SARS-CoV-2 |
Expression System | E. coli |
Tag | N-His-Avi |
Accession Number | P0DTC9 |
Synonyms | Nucleocapsid,NP,NC |
Construction | Gly243-Pro364 |
Protein Purity | > 95% as determined by Tris-Bis PAGE |
Molecular Weight | 17 kDa (predicted) same as Tris-Bis PAGE result. |
Endotoxin | < 1 EU/μg by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | Nucleocapsid protein (N) is the major viral structural component; its main function is to protect and encapsidate the viral RNA forming viral RNP complex. It is encoded by the S segment vRNA and is abundantly expressed in the cytoplasm of infected cells. |
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