PPM1A Protein, Mouse, Recombinant (His)

Catalog No. TMPY-01663

PPM1A Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 43.3 kDa and the accession number is P49443.

PPM1A Protein, Mouse, Recombinant (His)

Catalog No. TMPY-01663

PPM1A Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 43.3 kDa and the accession number is P49443.

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100 μg	570 €	7-10 days

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Product Information

Biological Activity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	PPM1A Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 43.3 kDa and the accession number is P49443.
Species	Mouse
Expression System	E. coli
Tag	C-His
Accession Number	P49443
Synonyms	protein phosphatase, Mg2+/Mn2+ dependent, 1A,MPPa-1,MMPa-2,AU017636,AI427932,2900017D14Rik,2310003C21Rik
Construction	A DNA sequence encoding the mouse PPM1A (NP_032936.1) (Met 1-Trp 382) was expressed, with a polyhistide tag at the C-terminus. Predicted N terminal: Met 1
Protein Purity	> 98 % as determined by SDS-PAGE
Molecular Weight	43.3 kDa (predicted); 43.3 kDa (reducing conditions)
Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 10 mM HEPES, 500 NaCl, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Protein phosphatase 1A (PPM1A / PP2CA) is an enzyme belonging to the PP2C family of Ser / Thr protein phosphatases. Members of PP2C family are negative regulators of cell stress response pathways and the MAP kinases and MAP kinase kinases. It has also been demonstrated to inhibit the activation of p38 and JNK kinase cascades. PPM1A dephosphorylates and promotes nuclear export of TGFβ-activated Smad2/3. Ectopic expression of PPM1A abolishes TGFβ-induced antiproliferative and transcriptional responses, whereas depletion of PPM1A enhances TGFβ signaling in mammalian cells. It has been demonstrated that PPM1A / PP2CA, through dephosphorylation of Smad2/3, plays a critical role in terminating TGFβ signaling. Overexpression of PPM1A is reported to activate the expression of the tumor suppressor gene TP53 / p53, which leads to cell apoptosis.