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NGF Protein, Human, Recombinant (HEK293)

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NGF Protein, Human, Recombinant (HEK293)

Catalog No. TMPY-06985

NGF Protein, Human, Recombinant (HEK293) is expressed in HEK293 mammalian cells. The predicted molecular weight is 24.8 kDa. and the accession number is P01138.

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Pack Size	Price	Availability	Quantity
100 μg	$136	In Stock

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Biological Description

Biological Information	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 5-20 ng/mL.
Description	NGF Protein, Human, Recombinant (HEK293) is expressed in HEK293 mammalian cells. The predicted molecular weight is 24.8 kDa. and the accession number is P01138.
Species	Human
Expression System	HEK293 Cells
Tag	Tag Free
Accession Number	P01138
Synonyms	NGFB,Beta-NGF,HSAN5,β-NGF,nerve growth factor (beta polypeptide),nerve growth factor (β polypeptide)
Construction	Human NGF (P01138, with mutation Arg 121 Gly) (Met1-Ala241)
Protein Purity	> 95 % as determined by SDS-PAGE.
Molecular Weight	The recombinant human NGF consists of 223 amino acids and predicts a molecular mass of 24.8 kDa.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. . Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth-stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein that homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. Also, previous studies indicated that NGF may also have an important role in the regulation of the immune system.