Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1-6 ng/mL.

NGF Protein, Mouse, Recombinant is expressed in CHO mammalian cells. The predicted molecular weight is 13.5 kDa and the accession number is P01139.

Mouse

CHO Cells

Ngfb,nerve growth factor (β polypeptide),nerve growth factor (beta polypeptide)

A DNA sequence encoding the mature form of mouse NGF (NP_001106168.1) (Ser 122-Gly 241) was expressed, with an initial Met at the C-terminus. Predicted N terminal: Met

> 96 % as determined by SDS-PAGE

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth-stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein that homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. Also, previous studies indicated that NGF may also have an important role in the regulation of the immune system.