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S100A5 Protein, Human, Recombinant (His)

Catalog No. TMPY-01931

S100A5 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 14.2 kDa and the accession number is B48219.

S100A5 Protein, Human, Recombinant (His)

S100A5 Protein, Human, Recombinant (His)

Catalog No. TMPY-01931
S100A5 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 14.2 kDa and the accession number is B48219.
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100 μg$6007-10 days
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Product Information

Biological Activity
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
S100A5 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 14.2 kDa and the accession number is B48219.
Species
Human
Expression System
E. coli
TagN-His
Accession NumberB48219
Synonyms
S100D,S100 calcium binding protein A5
Construction
A DNA sequence encoding the human S100A5 (P33763) (Met 1-Lys 110) was expressed, with a polyhistide tag at the N-terminus. Predicted N terminal: Met
Protein Purity
> 92 % as determined by SDS-PAGE
Molecular Weight14.2 kDa (predicted); 14 kDa (reducing conditions)
EndotoxinPlease contact us for more information.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 10% glycerol, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the?neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. Protein S100-A5, also known as Protein S-100D, S100 calcium-binding protein A5, S100A5 and S100D, is a member of the S100 family which contains two EF-hand domains. S100A5 is also a novel member of the EF-hand superfamily of calcium-binding proteins that is poorly characterized at the protein level. It is expressed in very restricted regions of the adult brain. From birth onwards, S100A5 remained a neuronal-specific protein, only located in a subpopulation of neurons in the spiral ganglion.

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