SAE1 Protein, Human, Recombinant

Catalog No. TMPY-03441

SAE1 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 38.6 kDa and the accession number is Q9UBE0.

SAE1 Protein, Human, Recombinant

Catalog No. TMPY-03441

SAE1 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 38.6 kDa and the accession number is Q9UBE0.

Pack Size	Price	Availability
50 μg	$228	In Stock
100 μg	$392	7-10 days
200 μg	$670	7-10 days
500 μg	$1,350	7-10 days

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COA User Guide of Recombinant Proteins

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Product Information

Biological Activity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	SAE1 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 38.6 kDa and the accession number is Q9UBE0.
Species	Human
Expression System	Baculovirus Insect Cells
Tag	Tag Free
Accession Number	Q9UBE0
Synonyms	UBLE1A,SUMO1 activating enzyme subunit 1,SUA1,HSPC140,AOS1
Construction	A DNA sequence encoding the human SAE1 (Q9UBE0) (Met1-Lys346) was expressed and purified. Predicted N terminal: Gly
Protein Purity	> 95 % as determined by SDS-PAGE
Molecular Weight	38.6 kDa (predicted); 39 kDa (reducing conditions)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM Nacl, pH 7.4, 10% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	SAE1 belongs to the ubiquitin-activating E1 family. It is a heterodimer that acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It functions as a UBLI E1 ligase mediating the ATP-dependent activation of UBL1. SAE1 binds with UBLE1A and UBLE1B to form a heterodimer which can bind UBL1. SAE1 also regulates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins.