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SARS-CoV-2 3CLpro/3C-like protease Protein (His & Avi)

Catalog No. TMPY-05727

3C-like protease (3CLpro) is the main protease of Humann Coronavirus. 3C-like protease (3CLpro) is a key enzyme, as it cleaves several sites to produce non-structural proteins that are essential for genome replication and Coronavirus virion production, such as an RNA-dependent RNA polymerase, a helicase, ribonucleases and 3CLpro itself, from two types of polyproteins (pp1a and pp1ab). SARS-CoV 3CLpro exists as a homodimer and each protomer has an active site. SARS-CoV-2 3CLpro/3C-like protease Protein (His & Avi) is expressed in E. coli expression system. The predicted molecular weight is 36.57 kDa and the accession number is YP_009725295.1.

SARS-CoV-2 3CLpro/3C-like protease Protein (His & Avi)

SARS-CoV-2 3CLpro/3C-like protease Protein (His & Avi)

Catalog No. TMPY-05727
3C-like protease (3CLpro) is the main protease of Humann Coronavirus. 3C-like protease (3CLpro) is a key enzyme, as it cleaves several sites to produce non-structural proteins that are essential for genome replication and Coronavirus virion production, such as an RNA-dependent RNA polymerase, a helicase, ribonucleases and 3CLpro itself, from two types of polyproteins (pp1a and pp1ab). SARS-CoV 3CLpro exists as a homodimer and each protomer has an active site. SARS-CoV-2 3CLpro/3C-like protease Protein (His & Avi) is expressed in E. coli expression system. The predicted molecular weight is 36.57 kDa and the accession number is YP_009725295.1.
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100 μg663 €7-10 days
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Product Information

Biological Activity
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
3C-like protease (3CLpro) is the main protease of Humann Coronavirus. 3C-like protease (3CLpro) is a key enzyme, as it cleaves several sites to produce non-structural proteins that are essential for genome replication and Coronavirus virion production, such as an RNA-dependent RNA polymerase, a helicase, ribonucleases and 3CLpro itself, from two types of polyproteins (pp1a and pp1ab). SARS-CoV 3CLpro exists as a homodimer and each protomer has an active site. SARS-CoV-2 3CLpro/3C-like protease Protein (His & Avi) is expressed in E. coli expression system. The predicted molecular weight is 36.57 kDa and the accession number is YP_009725295.1.
Species
SARS-CoV-2
Expression System
E. coli
TagN-His-Avi
Accession NumberYP_009725295.1
Construction
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) 3CL Protease (YP_009725295.1) (Ser3264-Gln3569) was expressed with a N-terminal polyhistidine tag followed by a MBP tag. Predicted N terminal: Met
Protein Purity
> 95 % as determined by SDS-PAGE.
Molecular Weight36.57 kDa (predicted)
EndotoxinPlease contact us for more information.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
3C-like protease (3CLpro) is the main protease of Humann Coronavirus. 3C-like protease (3CLpro) is a key enzyme, as it cleaves several sites to produce non-structural proteins that are essential for genome replication and Coronavirus virion production, such as an RNA-dependent RNA polymerase, a helicase, ribonucleases and 3CLpro itself, from two types of polyproteins (pp1a and pp1ab). SARS-CoV 3CLpro exists as a homodimer and each protomer has an active site.

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