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SHH Protein, Human, Recombinant

Catalog No. TMPJ-01127

SHH Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 19 KDa and the accession number is Q15465.

SHH Protein, Human, Recombinant

SHH Protein, Human, Recombinant

Catalog No. TMPJ-01127
SHH Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 19 KDa and the accession number is Q15465.
Pack SizePriceAvailabilityQuantity
10 μg$1297-10 days
50 μg$3907-10 days
500 μg$1,5307-10 days
1 mg$2,1807-10 days
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Product Information

Biological Activity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
SHH Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 19 KDa and the accession number is Q15465.
Species
Human
Expression System
E. coli
TagTag Free
Accession NumberQ15465
Synonyms
Sonic Hedgehog Protein,SHH,HHG-1
Amino Acid
Cys24-Gly197
Construction
Cys24-Gly197
Protein Purity
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
SHH Protein, Human, Recombinant
Molecular Weight19 KDa (reducing condition)
Endotoxin< 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PB, 100 mM NaCl, 1 mM DTT, pH 7.5.
Reconstitution
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background
Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases. Human SHH is expressed as a 45kDa precursor, and undergoes a series of processing during secretion. After the removal of the signal peptide, a protease within the C-terminal domain catalyzes the cleavage of SHH into a 20 kDa N-terminal signaling domain (SHH-N) and a 25 kDa C-terminal domain (SHH-C). SHH-N has the “all signaling” capability. SHH-N binds to the 12 pass transmembrane protein Patched (Ptc) on cell surface, which releases the repression of the activity of Smoothened (Smo), a G-protein coupled receptor, by Ptc.

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Keywords