1. Immobilized SLIT2 Protein, Human, Recombinant (His) (Cat#TMPY-07086) at 10 μg/mL (100 μL/well) can bind ROBO1 Protein, Human, Recombinant (hFc) (Cat#TMPY-06346), the EC50 is 60-180 ng/mL.
2. Loaded ROBO1 Protein, Human, Recombinant (hFc) (Cat#TMPY-06346) on proA Biosensor, can bind SLIT2 Protein, Human, Recombinant (His) (Cat#TMPY-07086) with an affinity constant of 42.8 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

SLIT2 Protein, Human, Recombinant (His) is expressed in HEK293 Cells. The accession number is O94813.

Human

HEK293 Cells

Slit-2,SLIL3

A DNA sequence encoding the Human SLIT2 (O94813) (Leu271-Ala480) was expressed with a polyhistidine tag at the C-terminus.

≥ 95% as determined by SDS-PAGE. ≥ 90% as determined by SEC-HPLC.

Please refer to the lot-specific COA.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.