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Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μg | $357 | 7-10 days | |
500 μg | $2,330 | 7-10 days |
Biological Information | Loaded Recombinant Human Thy1/CD90 Protein, His Tag on NTA Biosensor, can bind Recombinant Human Galectin-1 Protein with an affinity constant of 46.9 nM as determined in BLI assay (Routinely tested). |
Description | Thy1/CD90 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 14 kDa and the accession number is B0YJA4. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-His |
Accession Number | B0YJA4 |
Synonyms | Thy-1 cell surface antigen,CD90 |
Construction | The Human THY1 (NP_006279.2) (Met1-Cys130) was expressed with a polyhistidine tag at the C-terminus. |
Protein Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC. |
Molecular Weight | 14 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | Thy-1 membrane glycoprotein, also known as Thy-1 antigen, CD90 and THY1, is a cell membrane protein which contains 1 Ig-like V-type (immunoglobulin-like) domain. It is a glycophosphatidylinositol-linked glycoprotein expressed on the surface of neurons, thymocytes, subsets of fibroblasts, endothelial cells, mesangial cells and some hematopoietic cells. It has been identified on a variety of stem cells and at varying levels in non-lymphoid tissues such as on fibroblasts, brain cells, and activated endothelial cells. Thy-1 is evolutionarily conserved, developmentally regulated, and often has dramatic effects on cell phenotype. Thy-1 is a 25-37 kDa glycosylphosphatidylinositol (GPI)-anchored protein involved in T cell activation, neurite outgrowth, apoptosis, tumor suppression, wound healing, and fibrosis. To mediate these diverse effects, Thy-1 participates in multiple signaling cascades. Thy-1 is an important regulator of cell-cell and cell-matrix interactions, with important roles in nerve regeneration, metastasis, inflammation, and fibrosis. |
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