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TIGIT Protein, Rat, Recombinant (hFc)

Catalog No. TMPY-05744
TIGIT Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 40.02 kDa and the accession number is XP_008766987.1.
Pack SizePriceAvailabilityQuantity
100 μg$7007-10 days
500 μg$1,9507-10 days
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Product Information

Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized Nectin-2 Protein, Human, Recombinant (ECD, His Tag) at 2 μg/ml (100 μl/well) can bind TIGIT Protein, Rat, Recombinant (hFc Tag), the EC50 of TIGIT Protein, Rat, Recombinant (hFc Tag)is 60-300 ng/mL.
Description
TIGIT Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 40.02 kDa and the accession number is XP_008766987.1.
Species
Rat
Expression System
HEK293 Cells
TagC-hFc
Accession NumberXP_008766987.1
Synonyms
T cell immunoreceptor with Ig and ITIM domains,RGD1563191
Construction
A DNA sequence encoding the rat TIGIT (XP_008766987.1) (Met1-Ala138) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Ala 17
Protein Purity
> 90 % as determined by SDS-PAGE.
Molecular Weight40.02 kDa (predicted)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-1 and diminished the production of IL-12p4. Also, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint TargetsImmunotherapyTargeted Therapy

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