1. Immobilized Recombinant Human DCR3/TNFRSF6B Protein (Fc Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Mouse TL1A/TNFSF15 Protein (His Tag), Biotinylated, the EC50 is 5-15 ng/mL. 2. Loaded Biotinylated Recombinant Mouse TL1A/TNFSF15 Protein, His Tag on SA Biosensor, can bind Recombinant Human DcR3/TNFRSF6B Protein, hFc Tag with an affinity constant of 31.6 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

TL1A/TNFSF15 Trimer Protein, Mouse, Recombinant (His), Biotinylated is expressed in HEK293 Cells. The accession number is Q5UBV8.

Mouse

HEK293 Cells

Vegi,Tnlg1b,Tnfsf15,Tl1a,Tl1

A DNA sequence encoding the Mouse TNFSF15 (Q5UBV8) (Leu72-Leu251) was expressed with a polyhistidine tag at the N-terminus. The purified protein was biotinylated in vitro. This construct has a trimer design.

≥ 95% as determined by SDS-PAGE. ≥ 90% as determined by SEC-MALS.

Please refer to the lot-specific COA.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

TL1A, also known as TNFSF15, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It is specifically expressed in endothelial cells. TL1A also can be detected in monocytes, placenta, lung, liver, kidney, skeletal muscle, pancreas, spleen, prostate, small intestine and colon. TL1A is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6. It mediates activation of NF-kappa-B. It also inhibits vascular endothelial growth and angiogenesis (in vitro). TL1A promotes activation of caspases and apoptosis. It is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor.