TNF alpha Protein, Human, Recombinant

Catalog No. TMPY-00936

TNF alpha Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 17.5 kDa and the accession number is P01375.

TNF alpha Protein, Human, Recombinant

Catalog No. TMPY-00936

TNF alpha Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 17.5 kDa and the accession number is P01375.

Pack Size	Price	Availability
10 μg	$57	In Stock
20 μg	$82	In Stock
50 μg	$135	In Stock
100 μg	$197	In Stock

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COA User Guide of Recombinant Proteins

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Product Information

Biological Activity	1.Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL. 2.Captured Adalimumab on Anti-human IgG Fc via CM5 Chip can TNF-α with an affinity constant of 0.2843 nM as determined in a SPR assay(Routinely tested).
Description	TNF alpha Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 17.5 kDa and the accession number is P01375.
Species	Human
Expression System	E. coli
Tag	Tag Free
Accession Number	P01375
Synonyms	tumor necrosis factor,TNF-α,TNFSF2,TNF-alpha,TNFA,DIF
Construction	A DNA sequence encoding the human TNF-α soluble form (NP_000585.2) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus. Predicted N terminal: Met
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Molecular Weight	17.5 kDa (predicted); 17.5 kDa (reducing conditions)
Endotoxin	< 5 EU/mg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.TNF-alpha Protein & AntibodyCancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy