Measured by its ability to cleave a peptide substrate, Z-Gly-Gly-Arg-AMC TFA (Cat#T8556). Read at excitation and emission wavelengths of 380 nm and 460 nm (top read). The specific activity is 3861 pmol/min/µg.

Tissue plasminogen activator (tPA) is the predominant plasminogen activator present in the vascular and nervous systems.t tPA is not only neuroprotective for postnatal primary cortical neurons, but also that the predominant route for enhancing cell survival is via an mTORdependent mechanism.

Mouse

HEK293 Cells

t-PA,tPA,Reteplase,PLAT,Alteplase

Ser33-Gln559, which consists of two chains: chain A (Ser33-Arg308) and chain B (Ile309-Gln559)

> 95% as determined by Tris-Bis PAGE

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Tissue plasminogen activator (tPA) is the predominant plasminogen activator present in the vascular and nervous systems.t tPA is not only neuroprotective for postnatal primary cortical neurons, but also that the predominant route for enhancing cell survival is via an mTORdependent mechanism.