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TraY Protein, E. coli, Recombinant (His & SUMO)

Catalog No. TMPH-00724

TraY Protein, E. coli, Recombinant (His & SUMO) is expressed in E. coli expression system with N-6xHis-SUMO tag. The predicted molecular weight is 18.2 kDa and the accession number is P33789.

TraY Protein, E. coli, Recombinant (His & SUMO)

TraY Protein, E. coli, Recombinant (His & SUMO)

Catalog No. TMPH-00724
TraY Protein, E. coli, Recombinant (His & SUMO) is expressed in E. coli expression system with N-6xHis-SUMO tag. The predicted molecular weight is 18.2 kDa and the accession number is P33789.
Pack SizePriceAvailabilityQuantity
20 μg342 €20 days
100 μg644 €20 days
1 mg2.185 €20 days
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Product Information

Biological Activity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
TraY Protein, E. coli, Recombinant (His & SUMO) is expressed in E. coli expression system with N-6xHis-SUMO tag. The predicted molecular weight is 18.2 kDa and the accession number is P33789.
Species
E. coli
Expression System
E. coli
TagN-6xHis-SUMO
Accession NumberP33789
Synonyms
traY,Relaxosome protein TraY
Amino Acid
MKEPKSNIRKLIDIGGLIGR
Construction
1-20 aa
Protein Purity
> 85% as determined by SDS-PAGE.
Molecular Weight18.2 kDa (predicted)
FormulationTris-based buffer, 50% glycerol
Reconstitution
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background
Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site. Relaxosome formation requires binding of IHF and TraY to the oriT region, which then facilitates binding of TraI. Also positively regulates tra gene expression.

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Please read the User Guide of Recombinant Proteins for more specific information.