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TRIM21 Protein, Mouse, Recombinant (His & Avi), Biotinylated

Catalog No. TMPY-07233

TRIM21 Protein, Mouse, Recombinant (His & Avi), Biotinylated is expressed in Baculovirus-Insect Cells. The accession number is Q3U7K7.

TRIM21 Protein, Mouse, Recombinant (His & Avi), Biotinylated

TRIM21 Protein, Mouse, Recombinant (His & Avi), Biotinylated

Catalog No. TMPY-07233
TRIM21 Protein, Mouse, Recombinant (His & Avi), Biotinylated is expressed in Baculovirus-Insect Cells. The accession number is Q3U7K7.
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20 μg$588Backorder
100 μg$1,380Backorder
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Product Information

Biological Activity
1. Loaded Biotinylated Recombinant Mouse TRIM21 Protein, His Tag on SA Biosensor, can bind Herceptin with an affinity constant of 4.58 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
2. Loaded Biotinylated Recombinant Mouse TRIM21 Protein, His Tag on SA Biosensor, can bind Rituximab with an affinity constant of 8.76 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
3. Loaded Biotinylated Recombinant Mouse TRIM21 Protein, His Tag on SA Biosensor, can bind OKT3 with an affinity constant of 17.6 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
Description
TRIM21 Protein, Mouse, Recombinant (His & Avi), Biotinylated is expressed in Baculovirus-Insect Cells. The accession number is Q3U7K7.
Species
Mouse
Expression System
Baculovirus Insect Cells
TagN-His-Avi
Accession NumberQ3U7K7
Synonyms
Trim21,Ssa1,Ro52
Construction
A DNA sequence encoding the Mouse TRIM21 (NP_001076021.1) (Met1-Met462) was expressed with a N-terminal polyhistidine tag followed by an AVI tag.The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Protein Purity
≥ 80% as determined by SDS-PAGE.
Molecular Weight56.65 kDa (predicted); 49.4 kDa (reducing contition)
Endotoxin< 1.0 EU per μg protein as determined by the LAL method.
FormulationLyophilized from sterile 20 mM Tris, 500 mM NaCl, 20% Glycerol, 0.2 M Arg, 1 mM TCEP, pH 8.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Please refer to the lot-specific COA.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.

Dose Conversion

You can also refer to dose conversion for different animals. More

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Tech Support

Please read the User Guide of Recombinant Proteins for more specific information.