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Urokinase/uPA Protein, Human, Recombinant (Activated, His)

Catalog No. TMPY-07039

Urokinase/uPA Protein, Human, Recombinant (Activated, His) is expressed in HEK293 Cells. The accession number is P00749-1.

Urokinase/uPA Protein, Human, Recombinant (Activated, His)

Urokinase/uPA Protein, Human, Recombinant (Activated, His)

Catalog No. TMPY-07039
Urokinase/uPA Protein, Human, Recombinant (Activated, His) is expressed in HEK293 Cells. The accession number is P00749-1.
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Product Information

Biological Activity
Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The specific activity is >2000 pmoles/min/μg.
Description
Urokinase/uPA Protein, Human, Recombinant (Activated, His) is expressed in HEK293 Cells. The accession number is P00749-1.
Species
Human
Expression System
HEK293 Cells
TagC-His
Accession NumberP00749-1
Synonyms
URK,u-PA,UPA,QPD,BDPLT5,ATF
Construction
A DNA sequence encoding the human PLAU (NP_002649.1) (Met 1-Leu 431) with a C-terminal polyhistidine tag was expressed. The purified protein was activated by trypsin in vitro.
Protein Purity
≥ 97% as determined by SDS-PAGE. ≥ 90% as determined by SEC-HPLC.
Molecular Weight47.8 kDa (predicted); 19.1, 35.4 and 51 kDa (non-reducing contition, due to glycosylation and cleavage)
Endotoxin< 1.0 EU per μg of the protein as determined by the LAL method
FormulationLyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Please refer to the lot-specific COA.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
Plasminogen activator, urokinase, also known as PLAU and uPA, is a serine protease which converts plasminogen to plasmin, a broad-spectrum protease active on extracellular matrix (ECM) components. It is involved in complement activation, cell migration, wound healing, and generation of localized extracellular proteolysis during tissue remodelling, pro-hormone conversion, carcinogenesis and neoplasia. Like many components of the blood coagulation, fibrinolytic and complement cascades, uPA has a modular structure, including three conserved domains: a growth factor-like domain (GFD, residues 1-49), a kringle domain (residues 50-131), linked by an interdomain linker or "connecting peptide" (CP, residues 132-158) to the serine protease domain (residues 159-411). uPA and its receptor (uPAR) have been implicated in a broad spectrum of pathophysiological processes, including fibrinolysis, proteolysis, inflammation, atherogenesis and plaque destabilization, all of which are involved in the pathogenesis of MI (myocardial infarction). The role of uPA is not only linked to its action as an enzyme. In fact, the mere binding of uPA on the cell surface also brings about two events that broaden the spectrum of its biological functions: (1) a conformational change of the receptor, which, in turn, affects its interaction with other proteins; (2) a signal transduction which modulates the expression of apoptosis-related genes. Besides its applications as a thrombolytic agent and as a prognostic marker for tumors, uPA may provide the basis for other therapies, as the structure of the receptor-binding domain of uPA has become a model for the design of anti-cancer molecules. Because of the causal involvment of uPA in cancer invasion and metastasis, the blockade of uPA interactions and activity with specific inhibitors is of interest for novel strategies in cancer therapy.

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