α-CN-TO is a versatile dye utilized in biological experiments to help researchers observe cell structures, track and detect biomolecules, evaluate cell functions, distinguish cell types, study tissue pathology, and monitor microorganisms. Its applications span from basic scientific research to clinical diagnostics. Additionally, dyes like α-CN-TO are used in traditional fields such as textile dyeing and in emerging areas such as functional textile processing, food pigments, and dye-sensitized solar cells.