4-Nitro-7-piperazinobenzofurazan serves as a multifunctional dye, crucial in both scientific and industrial applications. As invaluable tools in biological experiments, dyes enable researchers to study cell structures, track biomolecules, assess cell functions, differentiate between cell types, detect biomolecules, analyze tissue pathology, and monitor microorganisms. Their utility extends beyond basic research into clinical diagnostics and various traditional uses including textile dyeing. Furthermore, dyes play significant roles in innovative sectors like functional textile processing, food coloring, and dye-sensitized solar cells.