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5-IAF

Name: 5-IAF
Brand: TargetMol
SKU: T14052
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No. T14052Cas No. 63368-54-7

Alias 5-Iodoacetamidofluorescein

5-IAF is a fluorescein derivative of indoleacetamide, a fluorescent probe that can be used to label proteins and others containing free thiols (cysteine side chains). 5-IAF can label the catalytic (alpha) subunit of dog kidney Na,K-ATPase.

5-IAF

Purity: 98%

Catalog No. T14052Alias 5-IodoacetamidofluoresceinCas No. 63368-54-7

Pack Size	Price	Availability
1 mg	$30	In Stock
5 mg	$68	In Stock
1 mL x 10 mM (in DMSO)	$129	In Stock

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Purity:98%

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COA

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity

Description	5-IAF is a fluorescein derivative of indoleacetamide, a fluorescent probe that can be used to label proteins and others containing free thiols (cysteine side chains). 5-IAF can label the catalytic (alpha) subunit of dog kidney Na,K-ATPase.
In vitro	8 µM GSH sample solution is derivatized with 8, 80, 200, 400, 800, 1600, 2000, 2500 µM 5-IAF, respectively. It was analyzed with CE-LIF, the highest signal intensity is obtained with 400 and 800 µM 5-IAF.[2]
Alias	5-Iodoacetamidofluorescein

Chemical Properties

Molecular Weight	515.25
Formula	C₂₂H₁₄INO₆
Cas No.	63368-54-7

Storage & Solubility Information

Storage

store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMF: 90.0 mg/mL (174.7 mM ), Sonication and heating to 60℃ are recommended.

DMSO: 45.0 mg/mL (87.3 mM), Sonication is recommended.

Solution Preparation Table

DMSO/DMF

	1mg	5mg	10mg	50mg
1 mM	1.9408 mL	9.7040 mL	19.4081 mL	97.0403 mL
5 mM	0.3882 mL	1.9408 mL	3.8816 mL	19.4081 mL
10 mM	0.1941 mL	0.9704 mL	1.9408 mL	9.7040 mL
20 mM	0.0970 mL	0.4852 mL	0.9704 mL	4.8520 mL
50 mM	0.0388 mL	0.1941 mL	0.3882 mL	1.9408 mL

DMF

	1mg	5mg	10mg	50mg
100 mM	0.0194 mL	0.0970 mL	0.1941 mL	0.9704 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

5-IAF