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EDANS

EDANS (EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid]) is a novel and quenched fluorogenic substrate for assaying retroviral protease by resonance energy transfer (RET).

Catalog No. TD0081Cas No. 50402-56-7

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Purity:99.73%

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COA HPLC HNMR

EDANS

Catalog No. TD0081Cas No. 50402-56-7

EDANS (EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid]) is a novel and quenched fluorogenic substrate for assaying retroviral protease by resonance energy transfer (RET).

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Pack Size	Price	Availability	Quantity
50 mg	$30	In Stock
1 mL x 10 mM (in DMSO)	$43	In Stock

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Product Introduction

Bioactivity

Description	EDANS (EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid]) is a novel and quenched fluorogenic substrate for assaying retroviral protease by resonance energy transfer (RET).
In vitro	EDANS is one of the most popular donors for developing FRET-based protease substrates and nucleic acid probes. EDANS is often paired with DABSYL or DABCYL in FRETbased probes. Its fluorescence is environment-sensitive.
Alias	EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid], 1,5-EDANS, EDANS acid [5-((2-Aminoethyl)amino)napht

Chemical Properties

Molecular Weight	266.32
Formula	C₁₂H₁₄N₂O₃S
Cas No.	50402-56-7

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 13.89 mg/mL (52.16 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.7549 mL	18.7744 mL	37.5488 mL	187.7441 mL
5 mM	0.7510 mL	3.7549 mL	7.5098 mL	37.5488 mL
10 mM	0.3755 mL	1.8774 mL	3.7549 mL	18.7744 mL
20 mM	0.1877 mL	0.9387 mL	1.8774 mL	9.3872 mL
50 mM	0.0751 mL	0.3755 mL	0.7510 mL	3.7549 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

inhibitfluorogenic substrateresonance energy transferRETEDANSretroviral proteaseInhibitor