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Mirin

Catalog No. T60273 CAS 299953-00-7

Mirin is a potent Mre11-Rad50-Nbs1 (MRN) complex inhibitor. Mirin prevents MRN-dependent activation of ATM (IC 50 =12 μM) without affecting ATM protein kinase activity, and it inhibits Mre11-associated exonuclease activity. Consistent with its ability to target the MRN complex, Mirin abolishes the G2/M checkpoint and homology-dependent repair in mammalian cells [1].

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Mirin, CAS 299953-00-7

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Biological Description

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Description

In vitro

Mirin effectively inhibits the phosphorylation of H2AX at an IC50 value of 66 μM and suppresses ATM-dependent phosphorylation of downstream targets Nbs1 and Chk2, as well as MRN-dependent autophosphorylation of ATM at Ser1981, following double-strand breaks (DSBs). Additionally, Mirin induces significant G2 phase arrest at both 50 μM and 100 μM concentrations. At doses ranging from 10-100 μM, Mirin disrupts homology-dependent DNA repair in TOSA4 cells, indicating its potential as a therapeutic agent in targeting DNA repair mechanisms [1]. Furthermore, BRCA2-deficient cells exhibit increased sensitivity to Mirin, highlighting its effectiveness as an Mre11 inhibitor [2].

Molecular Weight	220.25
Formula	C10H8N2O2S
CAS No.	299953-00-7

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Citations

1. Bi X, Zhang M, Zhou J, et al.Phosphorylated Hsp27 promotes adriamycin resistance in breast cancer cells through regulating dual phosphorylation of c-Myc.Cellular Signalling.2023: 110913.

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Keywords

Mirin 299953-00-7 inhibitor inhibit

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