Pregnenolone monosulfate sodium salt is a powerful neurosteroid, the main precursor of various steroid hormones including steroid ketones. Pregnenolone monosulfate sodium salt can protect the brain from cannabis intoxication. Pregnenolone monosulfate sodi

Pregnenolone likely acts as a signaling-specific negative allosteric modulator binding to a site distinct from that occupied by orthosteric ligands. Pregnenolone does not modify agonist binding but only agonist efficacy. The effect of THC is obviously attenuated when slices are pre-treated with Pregnenolone 100 nM (15.1±1.8 % of inhibition). These effects are likely due to a pre-synaptic action of Pregnenolone. Thus, Pregnenolone blocks the increase in the paired-pulse ratio induced by THC but does not modify either the amplitude or the decay time of miniature EPSC [1].

Administering Pregnenolone (2 and 4mg/kg) prior to each self-administration session effectively reduces WIN 55,212-2 consumption and lowers the break-point in progressive ratio schedules. Moreover, Pregnenolone doses ranging from 2-6 mg/kg inhibit THC-induced food consumption in Wistar rats and C57BL/6N mice, as well as mitigate THC-related memory deficits in mice, without altering these behaviors independently [1].