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Tetramethylrhodamine-5-iodoacetamide

Catalog No. T17060Cas No. 114458-99-0
Alias 5-TMRIA

Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) is a thiol-selective reactive dye used to non-specifically label proteins through cysteine residues and to covalently label DNA fragments [1].

Tetramethylrhodamine-5-iodoacetamide

Tetramethylrhodamine-5-iodoacetamide

Catalog No. T17060Alias 5-TMRIACas No. 114458-99-0
Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) is a thiol-selective reactive dye used to non-specifically label proteins through cysteine residues and to covalently label DNA fragments [1].
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Product Introduction

Bioactivity
Description
Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) is a thiol-selective reactive dye used to non-specifically label proteins through cysteine residues and to covalently label DNA fragments [1].
In vitro
Tetramethylrhodamine-5-iodoacetamide, at concentrations ranging from 0.5 to 7.5 mM, demonstrates varying effects on the fluorescence labeling of carbonic anhydrase and myoglobin. Initial observations reveal exclusive labeling of the carbonic anhydrase band at low dye concentrations (up to a 40:1 dye:thiol ratio). With increased concentrations, however, both carbonic anhydrase and the less prominent myoglobin band exhibit fluorescence labeling, indicating nonspecific interactions at these dye:thiol ratios[1].
Alias5-TMRIA
Chemical Properties
Molecular Weight569.39
FormulaC26H24IN3O4
Cas No.114458-99-0
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

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